Richard J. Miron - Understanding Platelet-Rich Fibrin

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Platelet concentrates have been used in medicine for over 20 years now, but the last 5 years have witnessed an explosion in research on platelet-rich fibrin (PRF) because of its ability to promote healing of both bone and soft tissue. This has led to a marked increase in our understanding of PRF therapy with respect to selection of appropriate centrifugation devices, impact of tube chemistry on clotting, the optimization of protocols to better concentrate PRF, and even the ability to extend the working properties of PRF from 2–3 weeks to 4–6 months using a simple heating process. Bringing together expert researchers and clinicians from various dental specialties, this book first explores the biology of PRF and then demonstrates its myriad clinical applications in periodontology, implant dentistry, oral and maxillofacial surgery, endodontics, facial esthetics, and medicine. The true value of this book lies in the blend of data and clinical application, so readers can feel confident knowing that the protocols recommended are fully supported by scientific evidence and demonstrated step by step by clinicians already using them in their daily practice. Even better, supplementary videos throughout showcase these procedures for better understanding. As Dr Robert E. Marx writes, «Understanding Platelet-Rich Fibrin is a book for this decade that transcends all specialties of dentistry and many of medicine,» so it's definitely one you'll want to read.

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Anika Dham

Research Student, Nova Southeastern University

Fort Lauderdale, Florida

Jonathan Du Toit, dds, msc

Department of Periodontics and Oral Medicine

Faculty of Health Sciences

University of Pretoria

Pretoria, South Africa

Meizi Eliezer, dds, msc, phd

Research Associate, Department of Periodontology

School of Dental Medicine

University of Bern

Bern, Switzerland

Masako Fujioka-Kobayashi, dds, phd

Professor, Department of Oral and Maxillofacial Surgery

School of Life Dentistry at Tokyo

The Nippon Dental University

Tokyo, Japan

Maria Elisa Galarraga-Vinueza, dds, msc, phd

Professor, School of Dentistry

Universidad de las Américas (UDLA)

Quito, Ecuador

Arun K. Garg, dmd

Private Practice Limited to Implantology

Miami, Florida

Stefan Gerber, md, dds, msc, phd

Assistant Professor, Department of Cranio-Maxillofacial Surgery

University of Bern

Bern, Switzerland

Ezio Gheno, dds, phd

Post-Graduation Program in Dentistry

Fluminense Federal University

Niterói, Rio de Janeiro, Brazil

Alfonso Gil, dds, msc

Resident, Fixed and Removable Prostho- dontics and Dental Material Science

University of Zurich

Zurich, Switzerland

Howard Gluckman, bds, mchd (omp)

Specialist in Periodontics, Implantology, and Oral Medicine

Director of Implant & Aesthetic Academy

Cape Town, South Africa

Reinhard Gruber, phd

Professor, Department of Oral Biology

Medical University of Vienna

Vienna, Austria

Thomas Lau Hansen, phd

Puremed

Roskilde, Denmark

Tommy Hardon, dvm

Head Veterinarian, Haslev Dyreklinik

Haslev, Denmark

David Lee Hill, dds, msc

Private Practice

Chapel Hill, North Carolina

Søren Jepsen, dds, msc, phd

Director of the Department of Periodontology

University of Bonn

Bonn, Germany

Valerie Kanter, dds, msc

Professor, Department of EndodonticsUniversity of California, Los Angeles

Los Angeles, California

Dwayne Karateew, dds, msc

Director of Program in Periodontics

University of Illinois at Chicago

Chicago, Illinois

Tomoyuki Kawase, dds, phd

Professor, Division of Oral Bioengineering

Institute of Medicine and Dentistry

Niigata University

Niigata, Japan

Johan Lenz, dvm

Veterinarian, Jonas Tornell Veterinär

Ängelholm, Sweden

Marius Leretter, dds, phd

University of Medicine and Pharmacy of Timişoara

Vice Dean of Dental School

Timişoara, Romania

Victoria Lima, dds, msc

Research Fellow, Division of Periodontics

Institute of Science and Technology

São Paulo State University (UNESP)

São Paulo, Brazil

Richard J. Martin, dds

Private Practice Limited to Oral and Facial Surgery

Lewisville, Texas

Yuriy May, dmd

Private Practice

Farmington, Connecticut

Brian Mealey, dds, ms

Professor and Graduate Program Director, Department of Periodontics

University of Texas Health Science Center at San Antonio

San Antonio, Texas

Jacob Coakley Meyer, dvm

Veterinarian, Charlottenlund Dyrehospital

Charlottenlund, Denmark

Richard J. Miron, dds, bmsc, msc, phd, dr med dent

Group Leader, The Miron Research Lab

Lead Educator, Advanced PRF Education

Venice, Florida

Department of Periodontology

University of Illinois at Chicago

Chicago, Illinois

Omid Moghaddas, dds, msc

Assistant Professor, Department of Periodontology

Dental Faculty, Tehran Medical Sciences

Islamic Azad University

Tehran, Iran

Naheed Mohamed, dmd, msd

Private Practice

Oakville, Ontario, Canada

Vittorio Moraschini, dds, msc, phd

Professor, Department of Oral Surgery

Dental School, Fluminense Federal University

Niterói, Rio de Janeiro, Brazil

Ana Paz, dds, ms

Private Practice

Lisbon, Portugal

Michael A. Pikos, dds, msc

Director, Pikos Institute

Tampa, Florida

Nikola Saulacic, dds, phd

Assistant Professor, Department of Cranio-Maxillofacial Surgery

University of Bern

Bern, Switzerland

Benoît Schaller, dr med, dr med dent

Professor, Department of Cranio- Maxillofacial Surgery

University of Bern

Bern, Switzerland

Anton Sculean, dmd, dr med dent, ms, phd

Professor and Executive Director and Chairman

Department of Periodontology

University of Bern

Bern, Switzerland

Senthil Selvan, dds

Director, Jeya Dental Clinic

Theni, India

Samer Shaltoni, dmd, ms

Private Practice Limited to Oral Surgery Buffalo Grove, Illinois

Yoshinori Shirakata, dds, phd

Associate Professor, Department of Periodontology

Kagoshima University Graduate School of Medical and Dental Sciences

Kagoshima, Japan

Miguel Stanley, dds

Private Practice

Lisbon, Portugal

Robert Talac, md, phd

Director, Renaxis Spine and Orthopedic Clinic

Houston, Texas

Mustafa Tunali, dds, phd

Professor, Department of Periodontology

Haydarpasa Training Hospital

Gulhane Military Medical Academy

Istanbul, Turkey

Delia Tuttle, dds, ms

Private Practice

Lake Elsinore, California

Hom-Lay Wang, dds, msd, phd

Professor and Director of Graduate Periodontics

Department of Periodontics and Oral Medicine

University of Michigan School of Dentistry

Ann Arbor, Michigan

Hudi Xu, dds, phd

Research Associate, Department of Dental Implantology

School of Stomatology

Wuhan University

Wuhan, China

Yufeng Zhang, md, dds, phd

Professor, Department of Dental Implantology

School of Stomatology

Wuhan University

Wuhan, China

Abbreviations

The abbreviations listed here are used throughout the book and are NOT always spelled out in the chapters for ease of reading.

ALP alkaline phosphatase
AM amniotic membrane
A-PRF advanced PRF
BoP bleeding on probing
BMP bone morphogenetic protein
CAF coronally advanced flap
CAL clinical attachment level
CBC complete blood count
CEJ cementoenamel junction
C-PRF concentrated-PRF
CTG connective tissue graft
DBBM deproteinized bovine bone mineral
DFDBA demineralized freeze-dried bone allograft
ECM extracellular matrix
EDTA ethylenediaminetetraacetic acid
EGF epidermal growth factor
EMD enamel matrix derivative
e-PRF extended-PRF
ePTFE expanded polytetrafluoroethylene
FDA US Food and Drug Administration
FDBA freeze-dried bone allograft
GBR guided bone regeneration
GF growth factor
H&E hematoxylin-eosin stain
hPDLC human periodontal ligament cell
H-PRF PRF obtained through horizontal centrifugation
IGF insulinlike growth factor
IL interleukin
i-PRF injectable-PRF
ISQ implant stability quotient
KTW keratinized tissue width
L-PRF leukocyte PRF
LPS lipopolysaccharide
LSCC low-speed centrifugation concept
mRNA messenger RNA
MRONJ medication-related osteonecrosis of the jaw
MSC mesenchymal stem cell
OFD open flap debridement
ONJ osteonecrosis of the jaw
PD probing depth
PDGF platelet-derived growth factor
PPE personal protective equipment
PPP platelet-poor plasma
PRF platelet-rich fibrin
PRGF plasma rich in growth factors
PRP platelet-rich plasma
PTFE polytetrafluoroethylene
RBC red blood cell
RBH residual bone height
RCF relative centrifugal force
RCT randomized controlled trial
rpm revolutions per minute
RT-PCR real-time polymerase chain reaction
SD standard deviation
SE standard error
SEM scanning electron microscopy
TGF-β transforming growth factor β
TMJ temporomandibular joint
TNF-α tumor necrosis factor α
T-PRF titanium-prepared PRF
VEGF vascular endothelial growth factor
WBC white blood cell

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