Biodiesel Technology and Applications

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Energy technologies have attracted great attention due to the fast development of sustainable energy. Biodiesel technologies have been identified as the sustainable route through which overdependence on fossil fuels can be reduced. Biodiesel has played a key role in handling the growing challenge of a global climate change policy. Biodiesel is defined as the monoalkyl esters of vegetable oils or animal fats. Biodiesel is a cost-effective, renewable, and sustainable fuel that can be made from vegetable oils and animal fats. Compared to petroleum-based diesel, biodiesel would offer a non-toxicity, biodegradability, improved air quality and positive impact on the environment, energy security, safe-to-handle, store and transport and so on. Biodiesels have been used as a replacement of petroleum diesel in transport vehicles, heavy-duty trucks, locomotives, heat oils, hydrogen production, electricity generators, agriculture, mining, construction, and forestry equipment.
This book describes a comprehensive overview, covering a broad range of topics on biodiesel technologies and allied applications. Chapters cover history, properties, resources, fabrication methods, parameters, formulations, reactors, catalysis, transformations, analysis, in situ spectroscopies, key issues and applications of biodiesel technology. It also includes biodiesel methods, extraction strategies, biowaste utilization, oleochemical resources, non-edible feedstocks, heterogeneous catalysts, patents, and case-studies. Progress, challenges, future directions, and state-of-the-art biodiesel commercial technologies are discussed in detail. This book is an invaluable resource guide for professionals, faculty, students, chemical engineers, biotechnologists, and environmentalists in these research and development areas.

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Table 1.1Some of the commonly used bacterial lipases for biodiesel production.

Enzyme Immobilized on Substrate Acyl acceptor Yield Reference
Burkholderia cepacia Lipase Hydrophobic silica monolith Jatropha oil Methanol 95% [125]
Hybrid matrix of alginate and κ-carrageenan Jatropha curcas L. oil Ethanol 100% [126]
κ-carrageenan Palm oil Methanol 100% [127]
Modified attapulgite Jatropha oil Methanol 94% [128]
SiO 2-PVA Babassu oil Ethanol 100 % [129, 130]
SiO 2-PVA Babassu oil Ethanol 100% [131]
Nb 2O 5 Babassu oil Ethanol 74.1% [132]
Pseudomonas cepacia Lipase Epoxy-acrylic resin Waste vegetable oil Ethanol 46–47% [133]
Phyllosilicate solgel matrix restaurant grease Methanol and Ethanol 98% [134]
Fe 3O 4nanoparticle biocomposite Soybean oil Methanol >88% [135]
Accurel Madhuca indica Ethanol 96% [136]
Protein-coated microcrystals Soybean oil Ethanol 98.93% [137]
Celite Jatropha oil Ethanol 98% [89]
Pseudomonas fluorescence Octyl-silica resin Babassu oil Ethanol 97.5% [85]
Hydrophobic sol-gel Soybean oil Methanol 65% [138]
Porous kaolinite particles Triolein Methanol and Ethanol 90% [139]
Asymmetric membrane Triolein Methanol 80% [140]

Table 1.2 Some of the commonly used fungal lipases for biodiesel production.

Enzyme Immobilized on Substrate Acyl acceptor Yield Reference
Candida antartica Lipase Activated textile cloth Waste cooking oil Methanol 91.08% [157]
Polyurethane foam Soybean oil Ethanol 81% [158]
Acrylic resin Sunflower oil Ethyl acetate 92.7% [78]
Soybean oil Methanol 83.31% [95]
Candida rugosa Lipase Microporous bio silica-polymer Scenedesmus quadricauda microalgal oil Methanol 96.4% [59]
Poly(styrenemethacrylic acid) microsphere Soybean oil Methanol 86% [159]
within an activated carbon as support Palm oil Methanol 70% [160]
Thermomyces lanuginosus Lipase Olive pomace Pomace oil Methanol 93% [161]
Phyllosilicate sol-gel matrix Grease Ethanol 80-90% [162]
Mesoporous polyhydroxybutyrate particles (PHB) Oleic acid Methanol and Ethanol 90% [163]
Toyopearl AF-amino-650M resin Babassu oil Ethanol 86.6% [164]

Table 1.3 Some examples commercial lipases commonly used for biodiesel production.

Enzyme Substrate Acyl acceptor Reaction yield Reference
Novozyme 435 Chlorella sp. KR-1 Dimethyl carbonate and methanol mixture 90% [145]
Sunflower oil Methanol, Absolute ethanol, 1-propanol >90% [146]
Oleic acid Ethanol, n-propanol, and n-butanol >90% [147]
Crude soybean oil Methanol 94% [148]
Soybean oil Ethyl acetate 63.3 % [149]
Soybean oil Ethanol [150]
Lipozyme TL IM Soybean oil Methanol >90% [151]
Crude palm oil Methanol 96.15% [90]
Waste cooking oil Methanol 92.8% [152]
Palm oil Oleyl alcohol 79.54% [153]
Corn oil Methanol 92% [154]
Lipozyme RM IM Crude rapeseed oil Monoacylglycerol 90% [155]
Sunflower oil Methanol >80% [82]
Castor oil Ethanol 98% [3]
Soybean oil deodorizer distillate Ethanol >88% [156]

Extracellular lipases are separated from broth containing lipase producing cells and after purification used as a catalyst in biodiesel production processes [55]. The way to purify extracellular lipases depends upon its structure and source organism [80]. Mostly extracellular lipases are used in the immobilized form for transesterification than as free lipases because of the low conversion rate and costly process [11]. Literature is full of different methods as well as materials used for immobilization of extracellular lipases. Main methods for immobilization involve cross linking, carrier binding and entrapment while the most commonly used materials for immobilization include silica, magnetic particles, and nanofibers or nanoparticles for carrier binding, alginate beads, gels, and silicon polymers for entrapment and glutaraldehyde for cross-linking [47]. The use of a suitable solvent in case of extracellular lipase is a key factor for high yield in transesterification as the use of unrelated solvent or absence of solvent results in very low yield [102, 165]. The use of extracellular lipase is also adapted because the use of intracellular lipase results in difficulties of extraction and purification of the final product [171]. Extracellular lipases are obtained from Candida guilliermondii , Burkholderia glumae , Pseudomonas aeruginosa , and Yarrowia lipolytica [176–179]. Table 1.4indicated a comparison between intracellular and extracellular lipases.

Table 1.4 Comparison between intracellular and extracellular lipase.

Intracellular lipase Extracellular lipase
Present inside the cell or linked to its walls (cell bound lipase) Separated from cells producing it
No need of isolation and purification steps Complex isolation and purification are required before using it for biodiesel production
Low conversion rate High conversion rate
Not analyzed by direct sampling Analyzed by direct sampling
Direct immobilization of lipase producing cells (whole-cell immobilization Purification is required before immobilization
Biodiesel production is cost-effective Biodiesel production is costly

1.9 Recombinant Lipases for Cost-Effective Biodiesel Production

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