Cheanyeh Cheng - Enzyme-Based Organic Synthesis

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Cheanyeh Cheng

Department of Chemistry

Chung Yuan Christian University

Chungli

Taiwan

This edition first published 2022

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Library of Congress Cataloging‐in‐Publication Data

Names: Cheng, Cheanyeh, author.

Title: Enzyme‐based organic synthesis / Cheanyeh Cheng, Department of Chemistry, Chung Yuan Christian University, Chungli, Taiwan.

Description: Hoboken, NJ : Wiley, 2022. | Includes bibliographical references and index.

Identifiers: LCCN 2021031980 (print) | LCCN 2021031981 (ebook) | ISBN 9781118027943 (hardback) | ISBN 9781118995143 (adobe pdf) | ISBN 9781118995150 (epub)

Subjects: LCSH: Enzymes–Synthesis. | Organic compounds–Synthesis. | Biocatalysis. | Enzymes–Biotechnology.

Classification: LCC TP248.E5 C44 2021 (print) | LCC TP248.E5 (ebook) | DDC 661/.805–dc23

LC record available at https://lccn.loc.gov/2021031980LC ebook record available at https://lccn.loc.gov/2021031981

Cover Design: Wiley

Cover Image: Courtesy of author

The first time I was introduced to the field of microbiology was during the year 1982–1987, when I studied in the United States in the Department of Chemical & Biochemical Engineering of the Graduate and Professional School of Rutgers University to pursue my PhD degree. I was surprised that bacteria can live in an environment without air and at a temperature much higher than room temperature as well as ferment glucose to acetic acid. This study ignited my interest in the research of microorganisms and enzymes. As soon as I finished my PhD study in 1987, I came back to my alma mater, Chung Yuan Christian University, and worked as an associate professor in the Department of Chemistry, the place where I obtained my BS degree in 1974. I decided to continue my PhD research work to study the enzymatic cellulose hydrolysis for producing glucose using raw materials such as waste paper, dead tree branch, or waste bamboo chopsticks and the enantioselective bioreduction of ketones catalyzed by whole yeast cells for producing chiral secondary alcohols. I also taught a course called bioorganic chemistry, which focuses on the chemo‐, stereo‐, and regioselective enzyme or whole microbial cell catalyzed organic synthesis.

Ten years ago, I received an invitation from Wiley to write this book. At that moment I did not realize it is a big challenge for me to write a comprehensive book concerning enzyme‐catalyzed organic synthesis using six classes of enzymes. With the kind of courage that “the newborn calf is not afraid of tigers,” I accepted this invitation and wrote a book writing proposal. Fortunately, my proposal was approved by reviewers and I immediately started writing this book. Then I found I cannot concentrate my mind on writing this book and it takes me a long time to finish a chapter due to my teaching loads, my research works, student and family affairs, and many other trivial things. In fact, I could put all my time and mind on writing this book only after my retirement from school three and half years ago. I really appreciate the tolerant heart of Wiley editor to allow me to finish this book in such a long time. I learned a lot from writing this book, which also opened a new vision for me in the field of microbes and enzyme‐catalyzed organic synthesis. I also deeply understood the meaning of the Chinese proverb “Live and Learn.” What I did and what knowledge I acquired in my 30 years of academic career is only a small part of the field, just like a drop in the ocean. However, I sincerely hope that through this book more people will be interested in the field of enzyme‐catalyzed organic synthesis.

Life originated from single‐cell microorganisms, and microorganisms that cannot be seen by the human eye have existed on Earth since prehistoric times. Enzymes catalyze diverse chemical reactions in microbial cells from time to time and silently participate in the progress of life. The life phenomena presented by the variety of chemistry involved in the microbial cells is like a solemn and brisk music suite of life. No one would have expected that the relationship between enzymes and the tiny universe of microorganisms is so close and inseparable. Microorganisms are also taken as a cell factory by scientists due to their ability to produce various kinds of useful chemicals for human. However, as a result of the division of labor in science today, chemists, biochemists, biologists, biomedical scientists, biochemical engineers, etc., each use their own specialized scientific expertise to explore this life community, which has led to the difficulty in communication and the inefficient integration among different academic disciplines. Therefore, one of the goals of this book is to enable researchers from different disciplines to communicate and gain consensus to achieve integration.