Tarso B. Ledur Kist - Open and Toroidal Electrophoresis

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Presents the theory and applications of Toroidal Capillary, Microchip, and Slab Electrophoresis to analytical chemists across a range of disciplines Written by one of the developers of Toroidal Capillary Electrophoresis (TCE), this book is the first to present this novel analytical technique, in detail, to the field of analytical chemistry.
The exact expressions of separation efficiency, resolution, peak capacity, and many other performance indicators of the open and toroidal layouts are presented and compared.
Featuring numerous illustrations throughout,
offers chapters covering: Solvents and Buffer Solutions; Fundamentals of Electrophoresis; Open Layout; and Toroidal Layout. Confronting Performance Indicators is next, followed by chapters on High Voltage Modules and Distributors; Heat Removal and Temperature Control; and Detectors. The book finishes with an examination of the applications of Toroidal Electrophoresis.
The first book to offer a detailed account of Toroidal Electrophoresis—written by one of its creators
Compares the toroidal layouts with the well-established open layouts of the three most used platforms (Capillary, Microchip, and Slab) Provides solutions to many of the experimental issues arising in electromigration techniques and discusses the voltage distributors and detectors that are compatible with the toroidal layouts Richly illustrated with a large number of useful equations showing the relationships between important operational parameters and the performance indicators 
is aimed at method developers and separation scientists working in clinical analysis, and food analysis, as well as those in pharmacology, disease biomarker applications, and nucleic acid analysis using the Capillary, Microchip, or slab Platform. It will also benefit undergraduate and graduate students of inorganic analytical chemistry, organic analytical chemistry, bioanalysis, pharmaceutical sciences, clinical sciences, and food analysis.

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5 Chapter 6Figure 6.1 Schematic view of four high voltage modules connected to the four...Figure 6.2 Schematic view of the switching sequence that must be used when f...Figure 6.3 Schematic view of the positions that must be used to energize the...Figure 6.4 Illustration of the vertical cut of a rotating high voltage distr...Figure 6.5 Top view of the static base картинка 37of Figure 6.4. This figure shows th...

6 Chapter 7Figure 7.1 Histogram showing the thermal conductivity of fused silica (blue)...Figure 7.2 Histogram showing the thermal conductivity of fused silica (blue)...Figure 7.3 Air in thermostated compartments flows orthogonally to the axial ...Figure 7.4 The velocity profile of a liquid coolant inside a polymer tube wh...Figure 7.5 Example of a cooling geometry that produces an approximately cons...Figure 7.6 A symmetrical cooling geometry similar to Figure 7.5, but compose...Figure 7.7 Example of a symmetrical cooling geometry using seven capillaries...Figure 7.8 A symmetrical cooling geometry consisting of a square capillary i...Figure 7.9 (A) Illustration of a symmetrical cooling geometry consisting of ...Figure 7.10 A symmetrical cooling geometry using square capillaries. The blu...Figure 7.11 Microchip with an ideal cooling design. The coolant (blue) circu...Figure 7.12 The wall of an ideal slab electrophoresis chamber. The separatio...

7 Chapter 8Figure 8.1 Electropherogram of a mixture of adenosine, AMP (degraded), ADP, ...Figure 8.2 Absorption spectra of the products of the fluoregenic reaction of...Figure 8.3 Absorption spectra of the products of the fluorogenic reaction of...Figure 8.4 Absorption spectra of compounds (mostly amines) labeled with 7-hy...Figure 8.5 Analysis of free proline and hydroxyproline in canned beef. The s...

8 Chapter 9Figure 9.1 Separation of L-phenylalanine from L-phenylalanine-ring-D5 using ...Figure 9.2 Number of theoretical plates calculated from L-phenylalanine-ring...Figure 9.3 Separation of ( картинка 38-hydroxybenzyl)methyltrimethylammonium and (2-hyd...

9 Appendix AFigure A.1 Illustration of the setup of a typical free flow electrophoresis ...Figure A.2 Illustration of the epitachophoresis setup recently described. It...

10 Appendix CFigure C.1 Simulation of a bi-dimensional free-solution electrophoresis of f...Figure C.2 Plot of equation C.10 showing the dynamic viscosity of pure water...Figure C.3 Plot of mobility ratio against temperature (in Celsius). The seco...Figure C.4 Velocity of the center of mass of a band during one period of a s...

11 Appendix DFigure D.1 A schematic representation of the negative surface density of cha...Figure D.2 These curves show the form of the electric potential function and...

12 Appendix EFigure E.1 Spatial concentration profile of an analytes at картинка 39. This is observ...

13 Appendix FFigure F.1 Illustration of the perspective view and frontal view of an ortho...

14 Appendix GFigure G.1 The effect of cyclic band compression on the variance of the band...Figure G.2 Effect of the multiplying factor картинка 40on plate height ( картинка 41). Four comp...

Guide

1 Cover

2 Table of Contents

3 Begin Reading

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Open and Toroidal Electrophoresis

Ultra-High Separation Efficiencies in Capillaries, Microchips, and Slabs

Tarso B. Ledur Kist

Department of Biophysics, Federal University of Rio Grande do Sul, Brazil

This edition first published 2020 2020 John Wiley Sons Ltd All rights - фото 42

This edition first published 2020

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