Tarso B. Ledur Kist - Open and Toroidal Electrophoresis

6 Chapter 8Table 8.1 Dependence of the performance indicators on the apparent mobility ( ) ...Table 8.2 Examples of non-fluorescent reagents that undergo fluorogenic reaction...

7 Appendix GTable G.1 The minimum and maximum values of , , and as a function of (time Table G.2 Performance indicators of the open layout (open) compared with toroida...

1 Chapter 1 Figure 1.1 Some neighbors of oxygen in the periodic table. The atomic number... Figure 1.2 Schematic representation of chloride anion solvation in water. Figure 1.3 Solvation of the sodium cation in water. Figure 1.4 c–pH diagram of citric acid species in an aqueous solution in the... Figure 1.5 p –pH diagram of citric acid species in an aqueous solution. The ... Figure 1.6 Buffer capacity of 0.1 M acetic acid (p ) in an aqueous solution ... Figure 1.7 Buffer capacity of 0.1 M Tris (p ) in an aqueous solution. Figure 1.8 Buffer capacity of 0.1 M -alanine (p and 9.6) in an aqueous sol... Figure 1.9 Buffer capacity of 0.1 M glycyl-aspartic acid (p , 4.45 and 8.6) ...

2 Chapter 2 Figure 2.1 The Cartesian coordinates used in this book for the microchanne... Figure 2.2 The cylindrical coordinates used in microtubes and flexible fus... Figure 2.3 Illustration of three flexible fused-silica microtubes (capillari... Figure 2.4 Ionizations and recombinations of a molecule along time, represen...Figure 2.5 Molar fraction of the species of the acid HA ( ) in relation to Figure 2.6 Molar fraction of the species of the conjugated acid BH +( ) ...Figure 2.7 Visualization of function , which is the solution of the diffusi...Figure 2.8 A separation simulation generated from a program written in a Qui...Figure 2.9 The same hypothetical run as in Figure 2.8, but this time it is s...Figure 2.10 End of the hypothetical run of Figure 2.8. (A) The separation pa...Figure 2.11 Average charge (ensemble average or time average) of -alanine, ...Figure 2.12 Average charge (ensemble average or time average) of 4-aminophen...Figure 2.13 Top view of the Poiseuille velocity profiles shown as level cont...Figure 2.14 Top view of the laminar fluid velocity profiles shown as level c...Figure 2.15 Top view of the fluid velocity profiles shown as level contours ...Figure 2.16 Fluid velocity profiles shown as level contours in Cartesian coo...Figure 2.17 Illustration of the electrical double layer at the capillary wal...Figure 2.18 EOF and Poiseuille velocity profiles for cylindrical microtubes ...Figure 2.19 Radial temperature profiles of a capillary that has 180 m OD, 5...Figure 2.20 Sample stacking illustrated with six snapshots. There are dozens...Figure 2.21 Nine snapshots illustrating an on-column band compression event....Figure 2.22 Variations of given as a function of the number of residues ( Figure 2.23 Example of a DNA sequencing run (electropherogram) using the ELF...Figure 2.24 Mobilities of three hypothetical analytes divided by the maximum...Figure 2.25 Productory of ( in the 0 to 14 pH range. The higher the product...Figure 2.26 Harmonic mean of the modulus of all pairs of with in the 0 t...Figure 2.27 Isoelectric focusing of amino acids, peptides, or proteins using...Figure 2.28 Separation of naphthalene-2,3-dicarboxaldehyde derivatized amino...Figure 2.29 Example of a DNA sequencing run (electropherogram) using the SE ...

3 Chapter 3Figure 3.1 Schematic illustration of the shape of the capillary platform, us...Figure 3.2 Schematic illustration of an array of capillaries mounted on a CE...Figure 3.3 Schematic illustration of the setup on a typical microchip platfo...Figure 3.4 Diagrammatic representation of the microfluidic operation procedu...Figure 3.5 The slab platform in the open layout, or open slab electrophoresi ...Figure 3.6 Perspective view of a vertical cut of an electrophoresis chamber ...Figure 3.7 Two barely resolved hypothetical bands or peaks (1 and 2). Their ...Figure 3.8 First derivatives (A) and second derivatives (B) of two unresolve...Figure 3.9 Resolution of partially overlapped bands/peaks. The thin curves a...Figure 3.10 The effect of asymmetry on the calculation of resolution between...

4 Chapter 4Figure 4.1 A) Photograph of the two ends of a 50 m ID and 365 m OD fused s...Figure 4.2 Illustration of the toroidal layout of the capillary platform usi...Figure 4.3 Illustration showing the same capillary toroid (T) of Figure 4.2,...Figure 4.4 Schematic view of the toroidal layout of the microchip platform s...Figure 4.5 Schematic view of the slab platform with a toroidal layout in the...Figure 4.6 Schematic view of the slab platform with a toroidal layout in the...Figure 4.7 Three examples of folding geometries: (A) toroid with a square cr...Figure 4.8 Schematic view of eight tori folded as ellipses. They are held in...Figure 4.9 Schematic view of the ideal geometry of a microhole etched onto t...