Genotyping by Sequencing for Crop Improvement

The DNA‐based molecular markers are widely recognized for their enormous potential in plant breeding and genetic studies. The past few years have seen remarkable developments in the field of molecular markers technology particularly with the emergence of NGS technologies. SNPs have become the choice of markers of present and future based on their genome‐wide abundance, high polymorphism, amenability to high‐throughput automation, and easier analysis. SNPs can be utilized in different genotyping platforms such as GBS, DArT, WGR, SNP arrays, and KASP. Any genotyping platform can be chosen based on the objective of the study and cost concerns. As an example, GBS, WGR, SNP arrays, and KASP can generate similar type of results for genetic diversity analysis; however, KASP and GBS could be cheaper than others. While the development of KASP will depend upon the availability of SNPs particularly in SNP databases, but GBS can be done without any previous information. It has been observed that these recent marker genotyping technologies have accelerated the crop improvement programs particularly in the identification and utilization of novel genes and QTLs.

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