Jane Flint - Principles of Virology

6 Before genome sequencing was possible, UV light was used to determine the sizes of vesicular stomatitis virus (VSV) mRNAs. In this experiment, viral particles are irradiated, and then mRNA synthesis is allowed to proceed. When UV light produces a lesion in the genome RNA, mRNA synthesis stops. Because of this property, synthesis of long mRNAs is more sensitive to UV light, while synthesis of short mRNAs is more resistant to UV light. The following UV sensitivity map was obtained:Use your knowledge of how VSV mRNAs are produced to explain this UV sensitivity map.

7 Influenza virus mRNAs are shorter than the genomic RNAs from which they are made. What is the mechanism for production of full-length copies of the genomic (–) strand RNAs for incorporation into newly synthesized virus particles?

8 Some RdRPs require a primer to initiate RNA synthesis. What are two different types of primer used for RNA synthesis?

9 The palm domain of poliovirus RdRP contains a GDD motif. Explain why changing D (Asp) to alanine produces an inactive polymerase that can no longer copy RNA templates.

Текст предоставлен ООО «ЛитРес».

Прочитайте эту книгу целиком, на ЛитРес.

Безопасно оплатить книгу можно банковской картой Visa, MasterCard, Maestro, со счета мобильного телефона, с платежного терминала, в салоне МТС или Связной, через PayPal, WebMoney, Яндекс.Деньги, QIWI Кошелек, бонусными картами или другим удобным Вам способом.